3h2 2d7 (Developmental Studies Hybridoma Bank)
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Structured Review
Developmental Studies Hybridoma Bank
3h2 2d7
3h2 2d7, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3h2+2d7/anti-Synaptotagmin/bio_rxiv__2025__04__29__651232-233-28-30
Average 94 stars, based on 17 article reviews
3h2 2d7, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3h2+2d7/anti-Synaptotagmin/bio_rxiv__2025__04__29__651232-233-28-30
Average 94 stars, based on 17 article reviews
3h2 2d7 - by Bioz Stars,
2026-09
94/100 stars
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Staining:Article Title: Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals Article Snippet: Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI), captured with a Leica SP5 confocal microscope, and processed with Fiji ( ). .. For NMJ staining, third-instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( Article Title: The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance Article Snippet: .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBST (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( Article Title: Ythdf is a N6-methyladenosine reader that modulates Fmr1 target mRNA selection and restricts axonal growth in Drosophila. Article Snippet: Images were acquired on a Leica TCS SP8 laser scanning confocal microscope or on a Leica DM6000CS confocal microscope and processed using FijiTM software. .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with the following primary antibodies: mouse anti-synaptotagmin, 1:200 ( Article Title: Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals Article Snippet: Nuclei were stained with DAPI and captured with Leica SP5 confocal and processed with FIJI . .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min . Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( Article Title: The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance Article Snippet: .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( Incubation:Article Title: Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals Article Snippet: Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI), captured with a Leica SP5 confocal microscope, and processed with Fiji ( ). .. For NMJ staining, third-instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( Article Title: The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance Article Snippet: .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBST (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( Article Title: Tissue specific function of the Drosophila 7SK snRNP in controlling motoneuron synaptic growth Article Snippet: .. The preparations were transferred to 35mm dish with silicone surface and washed six times with PBST (PBS+0.5% Triton- X) for 30 min each wash. Primary antibody anti-Syt 1:200 ( Article Title: Ythdf is a N6-methyladenosine reader that modulates Fmr1 target mRNA selection and restricts axonal growth in Drosophila. Article Snippet: Images were acquired on a Leica TCS SP8 laser scanning confocal microscope or on a Leica DM6000CS confocal microscope and processed using FijiTM software. .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with the following primary antibodies: mouse anti-synaptotagmin, 1:200 ( Article Title: Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals Article Snippet: Nuclei were stained with DAPI and captured with Leica SP5 confocal and processed with FIJI . .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min . Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( Article Title: The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance Article Snippet: .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 ( |
